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KMID : 1134820120410101417
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 10 p.1417 ~ p.1422
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Enzymatic Production of Alkyl β-Glucoside Based on Transglycosylation Activity of Celluclast
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Yong Hwan-Ung
Kim Seon-Mi
Shim Jae-Hoon
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Abstract
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Alkyl glucosides were synthesized using the transglycosylation reaction of Celluclast, the cellulase from Trichoderma reesei, with cellobiose and various alcohols. Glucose as a by-product of the reaction was removed using the immobilized yeast system. Among the alkyl glucoside products, the acceptor products of methanol and ethanol were confirmed as methyl β-D-glucopyranoside and ethyl β-D-glucopyranoside via MALDI-TOF MS and enzymatic analysis. Optimal yields of methyl β-glucoside and ethyl β-glucoside were 65.3% (mol/mol) and 59.0% (mol/mol), respectively, based on cellobiose consumed.
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KEYWORD
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transglycosylation, alkyl β-glucoside, yeast-immobilization
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